Batch CPC/CCC

Centrifugal Partition Chromatography (CPC) also known as Counter Current Chromatography (CCC) is a silica-free liquid-liquid chromatographic technique for preparative and industrial scale purifications. Two non-miscible liquid phases are used, one as the mobile phase or the eluent, and the other as the stationary phase maintained by a centrifugal field. The affinity of the solute for each phase, measured by their partition coefficient, gives their order of elution.

CPC/CCC could be used for wide range of applications such as synthetic compound purification, natural extract fractionation, natural compound purification, proteins separations, ...

For more details on this technology, click here to download the Armen brochure "Introduction to CPC/CCC"...

Advantages of CPC/CCC :
- Liquid chromatography without silica (no solid support, no silica to recycle)
- Low solvent consumption
- High flow rate for low run time
- High performances. Purity >99%, Recovery >90%
- No sample loss, no denaturation, no irreversible adsorption of the sample
- One column for all applications, from petroleum extract up to proteins purification

Application notes :
- Preparative Gingerol purification from natural extract by CPC
- Multi-dual CPC mode for Coumarins purification from Mesua elegans
- Preparative purification from Garcinia mangostana, CPC-LC-MS coupling
- Proteins purification by CPC
- Preparative Anthocyanins purification by CPC
- Purification of crown ethers by CPC

Armen "Spot CPC" systems :
Considering all benefits of this technique, Armen Instrument develops the range "Spot CPC" (SCPC), specially designed for high performance and scalability. From simple column to complete automated LC purification solution, all "SCPC" columns are available with or without dedicated integrated pump. The systems could be controlled directly through built-in keyboard (with LCD screen) or via "Armen Glider CPC" software installed on a computer.
The combination of "SCPC" column along "Spot Prep II" system and "Armen Glider CPC" software creates the most advanced purification tools efficient for all kind of applications.


SCPC-50

> Semi-preparative to Preparative CPC system

> 50 mL column capacity

> Injection range : Up to 0.5 g

> Typical Flow rate : Up to 15 mL/min for fast separation

> Controllable with "Spot Prep II" for automated, reliable and efficient purification

See the product


SCPC-100

> Semi-preparative to Preparative CPC system

> 100 mL column capacity

> Injection range : Up to 1 g

> Typical Flow rate : Up to 15 mL/min for fast separation

> Controllable with "Spot Prep II" for automated, reliable and efficient purification

See the product


SCPC-250

> Preparative CPC system

> 250 mL column capacity

> Injection range : Up to 6 g

> Typical Flow rate : Up to 15 mL/min for fast separation

> Controllable with "Spot Prep II" for automated, reliable and efficient purification

See the product


SCPC-1000

> Preparative to Pilot CPC system

> 1 L column capacity

> Injection range : Up to 30 g

> Typical Flow rate : Up to 50 mL/min for fast separation

> Controllable with "Spot Prep II" for automated, reliable and efficient purification

See the product


SCPC-100+1000

> Semi-Preparative to Pilot CPC system

> 2 columns in one box : 100 mL and 1 L capacities

> Injection ranges : Up to 1 g (on 100 mL column)
and Up to 30 g (on 1 L column)

> Typical Flow rates : Up to 15 mL/min (on 100 mL column)
and Up to 50 mL/min (on 1 L column)

> Controllable with "Spot Prep II" for automated, reliable and efficient purification

See the product


SCPC-250+1000

> Semi-Preparative to Pilot CPC system

> 2 columns in one box : 250 mL and 1 L capacities

> Injection ranges : Up to 6 g (on 250 mL column)
and Up to 30 g (on 1 L column)

> Typical Flow rates : Up to 15 mL/min (on 250 mL column)
and Up to 50 mL/min (on 1 L column)

> Controllable with "Spot Prep II" for automated, reliable and efficient purification

See the product